Journal: Cell Metabolism

Article Title: Mosaic dysfunction of mitophagy in mitochondrial muscle disease

doi: 10.1016/j.cmet.2021.12.017

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Beclin 1 , StressMarq , Cat# SPC-600D.

Techniques: Plasmid Preparation, Recombinant, Avidin-Biotin Assay, Blocking Assay, Software, Microscopy, Laser-Scanning Microscopy

Journal: BMC Cancer

Article Title: Differences in regulation of tight junctions and cell morphology between VHL mutations from disease subtypes

doi: 10.1186/1471-2407-9-229

Figure Lengend Snippet: Knockdown of p27 disrupts VHL-mediated tight junction formation . (A) RCC10 cells stably expressing WT pVHL 30 were infected with retroviruses containing shRNA constructs directed at luciferase as control (sh Luc) or p27 (sh p27). Approximately 2 weeks post-infection, cell lysates were equally loaded and subjected to p27 and α-tubulin western blotting. (B) RCC10 WT VHL cells infected with shRNA to luciferase or p27 were grown to confluence on collagen I and photographed by digital phase microscopy (original magnification of 100×). (C) RCC10 WT VHL cells infected with shRNA to luciferase or p27 were grown to confluence on coverslips and immunostaining for ZO-1 (left panels) was performed (original magnification of 1000×). DAPI labeled nuclei of corresponding cells are also shown (right panels). (D and E) 786-O cells stably expressing pVHL 19 were infected and analyzed as in (A) and (C).

Article Snippet: Rabbit anti-ZO-1 (Mid) was from Zymed.

Techniques: Stable Transfection, Expressing, Infection, shRNA, Construct, Luciferase, Western Blot, Microscopy, Immunostaining, Labeling

Journal: BMC Cancer

Article Title: Differences in regulation of tight junctions and cell morphology between VHL mutations from disease subtypes

doi: 10.1186/1471-2407-9-229

Figure Lengend Snippet: Contrasting regulation of tight junctions among VHL mutants of different disease types in RCC10 . RCC10 cells lines (as indicated; all created by retroviral infection) were grown to confluence on coverslips and immunostaining for ZO-1 (left panels) was performed (original magnification of 1000×). DAPI labeled nuclei of corresponding cells are also shown (right panels).

Article Snippet: Rabbit anti-ZO-1 (Mid) was from Zymed.

Techniques: Infection, Immunostaining, Labeling

Journal: BMC Cancer

Article Title: Differences in regulation of tight junctions and cell morphology between VHL mutations from disease subtypes

doi: 10.1186/1471-2407-9-229

Figure Lengend Snippet: Contrasting levels of ZO-1 fragments among VHL mutants of different disease types in RCC10 . RCC10 cells lines (as indicated; all created by retroviral infection) were grown to confluence. Cell lysates were prepared and equally loaded and separated by SDS-PAGE. Western blots were performed for ZO-1 and α-tubulin. Arrows indicate ZO-1 immunoreactive proteins.

Article Snippet: Rabbit anti-ZO-1 (Mid) was from Zymed.

Techniques: Infection, SDS Page, Western Blot

Journal: BMC Cancer

Article Title: Differences in regulation of tight junctions and cell morphology between VHL mutations from disease subtypes

doi: 10.1186/1471-2407-9-229

Figure Lengend Snippet: Tight junctions are affected by levels of HIF-2α in 786-O . (A) Parental 786-O cells (786-O), 786-O cells stably transfected with VHL (VHL +), and 786-O cells infected with an empty retrovirus (pSuperRetro) or infected with HIF-2α shRNAs (HIF2α shRNA), as described in , were grown for 1 week past confluence on collagen I coated culture dishes. Cell lysates were prepared, equally loaded, and western blotted for HIF-2α, cyclin D1, p27, α-tubulin and ZO-1 (both upper and lower immunoreactive species that were seen in Figure 9). (B) Indicated cell lines that were assayed in (A) were grown to confluence on coverslips and immunostained for ZO-1 (left panels; original magnification of 1000×). DAPI labeled nuclei of corresponding cells are also shown (right panels). (C) Indicated cell lines that were assayed in (A) and (B) were grown to confluence on collagen I and photographed by digital phase microscopy (original magnification of 100×).

Article Snippet: Rabbit anti-ZO-1 (Mid) was from Zymed.

Techniques: Stable Transfection, Transfection, Infection, shRNA, Western Blot, Labeling, Microscopy

Journal: Molecular cell

Article Title: MYC assembles and stimulates topoisomerases 1 and 2 in a “topoisome”

doi: 10.1016/j.molcel.2021.11.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-c-MYC (mid-ter) was from Novus (NBP2–49201).

Techniques: Recombinant, Control, Affinity Purification, Virus, Cell Culture, Protease Inhibitor, Magnetic Beads, Transfection, Plasmid Preparation, Titration, Staining, Thin Layer Chromatography, SYBR Green Assay, In Situ, Purification, Sample Prep, Sequencing, RNA Sequencing, Cloning, CRISPR, Inverse PCR, Expressing, Software